Composite

Part:BBa_K5520011:Design

Designed by: Ruyi Shi   Group: iGEM24_Ulink-SZ   (2024-09-29)


pT7-LacO-His- CsnBP115A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 844
    Illegal NotI site found at 804
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 813
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 153
    Illegal AgeI site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We aimed to investigate the relationship between the structure and function of chitosanase and to produce chitooligosaccharides with a defined degree of polymerization. To achieve this, amino acid mutation at site 115 was engineered to enhance enzymatic activity and to generate more uniform products.

Source

Escherichia coli and Bacillus pumilus

References